Experiment Session Notes

• Mouse ID: 820454
• Date: 2025_11_04
• Experimenter: @ryan-gillis
• Rig / Setup ID: NP1
• Stimulus version: Openscope_P3_v52 EPHYS_SESSION1_PROD_P3_SENSORYMOTOR
• Protocol followed: extracellular electrophysiology
• Notes & Issues:

| Probe | Target |

| Probe A | ACA |

| Probe B | Visa |

| Probe C | Visp |

| Probe D | Vislm |

| Probe E | Mop |

| Probe F | ACA Mos |

- first instance of using Openscope_P3_v52
-mouse had silicone plug removal the previous Friday (membrane regrowth expected)
- restarted all computers prior to putting the mouse on the rig
- Ryan performed the insertion, Casey was shadowing and assisting
- coated probes (5x dips) in CM-DiI
- agarose + silicone oil application went smoothly
- targeting went okay, lots of membrane on all probes
- all 6 probes inserted 
- settled probes for 30 minutes after reaching 3000 micron depth
- minor foam in corner of eye at 01:15, clears up a bit 
- pupil never obscured 
- Mouse walked a little (intermittent), no running. Stationary most of the experiment 
- First recording with new optic fiber mounts
    -frontal fiber positioned between probes E and F on tailcone (previously between F and A)

Data Access

• DANDI Repository: The data from this experiment has been uploaded to the DANDI Archive and is available at: DANDI:001637/sub-820454

• Data Format: Data is stored in Neurodata Without Borders (NWB) format.

• Code Ocean: Raw data (unpackaged and largely unprocessed) can be accessed on Allen Institute's Code Ocean Public Collection here

• Analysis Code: To explore the structure of the NWB file and validate its contents, you can use the intro_to_ephys_nwbs script in our repository.