Experiment Session Notes

• Mouse ID: 820459
• Date: 2025-11-10
• Experimenter: @ryan-gillis
• Rig / Setup ID: NP1
• Stimulus version: Openscope_P3_v52 EPHYS_SESSION1_PROD_P3_SENSORYMOTOR
• Protocol followed: extracellular electrophysiology
• Notes & Issues:

| Probe | Target |

| Probe A | ACA |

| Probe B | Visa |

| Probe C | Visp |

| Probe D | Vislm |

| Probe E | Mop |

| Probe F | ACA Mos |

- restarted all computers prior to putting the mouse on the rig
- Ryan performed the insertion, Casey was shadowing and assisting
- coated probes (5x dips) in CM-DiI
- agarose + silicone oil application went smoothly
- grounding issues before recording, troubleshooting added 10-15 minutes of rig time for mouse
- targeting went well (no membrane)
- all 6 probes inserted
-Blood obscuring vasculature on hole B (from plug removal -Casey)
-settled probes for 30 minutes after reaching 3000 micron depth
- Mild eye foam throughout experiment (in corner of eye)
    -Ring of foam appears at 01:20:00
    -pupil never obscured
-Mouse walked a little, no running. Stationary most of the experiment
-First recording with new optic fibers, mounted in same position as previous week
    -power readings tested higher, visually looked bright under scope
- brain visually healthy post experiment other than hole B

Data Access

• DANDI Repository: The data from this experiment has been uploaded to the DANDI Archive and is available at: DANDI:001637/sub-820459

• Data Format: Data is stored in Neurodata Without Borders (NWB) format.

• Code Ocean: Raw data (unpackaged and largely unprocessed) can be accessed on Allen Institute's Code Ocean Public Collection here

• Analysis Code: To explore the structure of the NWB file and validate its contents, you can use the intro_to_ephys_nwbs script in our repository.