Experiment Session Notes

• Mouse ID: 776270
• Date: 2025-07-02
• Experimenter: @amshelton @jeromelecoq
• Rig / Setup ID: SLAP2
• Stimulus version: https://raw.githubusercontent.com/AllenNeuralDynamics/openscope-community-predictive-processing/eb4f723e4dbb7725d0e0f65a45f615072f8b69d8/code/stimulus-control/src/Standard_oddball_slap2_version2.bonsai NOTE: We fixed a bug in the oddball block to make sure standard orientation alternated. Not On github yet.
• • Protocol followed: Glutamate (Green fluorophore) imaging + Calcium (iRGECO1a red fluorophore) imaging, 65 min experiment

• Notes & Issues:

  • Same experiment as last time. We record behavior videos (eye, face) with HARP timestamps aligned with the other events. Body camera was turned off as we are having file closing issues for all 3 cameras at the same time. We will fix later on.
  • SLAP2 was ran with SLAP trials to avoid analysis pipeline issues (30 seconds each, longer than before).
  • Screen is Gamma calibrated.
  • This design : https://github.com/AllenNeuralDynamics/openscope-community-predictive-processing/discussions/98
  • Orientation 1 : 60 trials
  • Oddball block 1 : Standard 0deg (1443 trials), 45 and 90 oddballs, omission (30 trials) shuffled, Oddball occurs on average every 11s
  • Orientation 2 : 33 trials
  • Oddball block 2 : Standard 0deg (1443 trials), 0 and 45 oddballs, omission (30 trials) shuffled, Oddball occurs on average every 11s
  • Orientation 3 : 33 trials
  • Nb Receptive fields : 10

  Total Expected duration = 65 min - Mouse is labelled with iGluSnFR4f and jRGECO1a both with viruses injection. We will image iGluSnFR4f and jRGECO1a both with two photon. Expression is rather sparse. - BEAUTFILL CELL! - Andrew circled the soma, a few proximal dendrites and an apical dendrites. So this experiment should yield calcium (red) + synaptic inputs (green). - DMD 1 is apical. DMD2 is proximal - Head fixation started at about 11:10 - Experiment started at about 11:50 - We ended exactly on time at 1:00 No sign of stress. No issues