Experiment Session Notes

• Mouse ID: 794237
• Date: 2025-05-08
• Experimenter: @Seyedolmohadesin @rcpeene @jeromelecoq @amshelton
• Rig / Setup ID: SLAP2
• Stimulus version: 523741b57359b8ad4ca7963611473fbb195ad010
• Protocol followed: Glutamate imaging, 50 min experiment

• Notes & Issues:

  • Similar experiment as last time. This time we record all 3 behavior videos (eye, face, body) with HARP timestamps aligned with the other events.

  • Baseline: 2405 -> NbBaselineGrating * (1 * (0.343+0.343)) -> 27.49 min

  • Mismatch: 40 -> NbMismatchPerCondition * 4 * (0.343+0.343)) -> 1.829 min of oddballs
  • Mismatch during orientation tuning: 40 -> 14.63 min
  • Receptive field: 10 -> NbReceptiveFieldRepeats * ((0.25) * 149) -> 6.2 min of RF

  Total Expected duration = 50.16 min Oddball occurs on average every 11s

  • Head fixation started at 13:55
  • Lot of issues with alignment of the eye tracking cameras. We managed at 14:25 to align the camera.
  • There is a fair amout of 2p cross talk to the behavior cameras. We will need to add a filter to remove it.
  • Otherwise all 3 cameras are functions.
  • Andrew is positioning imaging of dendrites at 2:40.
  • Maedeh weighting on choices of dendrites.
  • Experiment started at about 2;50.
  • Apical dendrites is imaged in DMD2.

  • Next experiment we will add filter to remove cross talk from 2p and control stimulus screen cross talk.

  • Experiment went smoothly. We ended on time at 3:42

  • Reviewing behavior videos show no signs of stress.

Data Access

• DANDI Repository: The data from this experiment has been uploaded to the DANDI Archive and is available at: DANDI:001424/sub-794237

• Data Format: Data is stored in Neurodata Without Borders (NWB) format.

• Analysis Code: To explore the structure of the NWB file and validate its contents, you can use the validate_nwb_slap2.py script in our repository.

Disclaimer: Please note that this is an early-stage data release. The data organization, metadata structure, and analysis tools are still being refined. If you encounter any issues or have questions about the data, please open an issue in the GitHub repository.