Experiment Session Notes

• Mouse ID: 796630
• Date: 2025-08-25
• Experimenter: @amshelton @jeromelecoq
• Rig / Setup ID: SLAP2
• Stimulus version: https://raw.githubusercontent.com/AllenNeuralDynamics/openscope-community-predictive-processing/eb4f723e4dbb7725d0e0f65a45f615072f8b69d8/code/stimulus-control/src/Standard_oddball_slap2_version2.bonsai NOTE AGAIN (Forgot to commit): We fixed a bug in the oddball block to make sure standard orientation alternated. Not On github yet. The code fix bug with camera file closure and fake initial pulses sent to SlAP.
• • Protocol followed: Glutamate (Green fluorophore) imaging, 65 min experiment

• Notes & Issues:

  • Same experiment as last time. We record behavior videos (body, eye, face) with HARP timestamps aligned with the other events.
  • SLAP2 was ran with SLAP trials to avoid analysis pipeline issues (30 seconds each, longer than before).
  • Screen is Gamma calibrated.
  • This design : https://github.com/AllenNeuralDynamics/openscope-community-predictive-processing/discussions/98
  • Orientation 1 : 60 trials
  • Oddball block 1 : Standard 0deg (1443 trials), 45 and 90 oddballs, omission (30 trials) shuffled, Oddball occurs on average every 11s
  • Orientation 2 : 33 trials
  • Oddball block 2 : Standard 0deg (1443 trials), 0 and 45 oddballs, omission (30 trials) shuffled, Oddball occurs on average every 11s
  • Orientation 3 : 33 trials
  • Nb Receptive fields : 10

  Mouse head fixed at 2:17PM

  Total Expected duration = 65 min

  Male mouse is labelled with 4E12 ExP32222-hSyn-Flex-iGluSnFR4.v9601.NGR + 2E12 pAAV.CAG.Flex.RCAMP3.WPRE.SV40 + 6E8 AAV9-CamKII0.4-Cre-SV40 injected on 4/1/2025 in V1, DOB 01/21/2025 We will image GluSNF4 with two photon.

  Andrew circled few proximal dendrites and apical dendrites. Some miscommunication occured and we did not circle soma.

  DMD 1 is superficial - 85um . DMD2 is deeper - 140um Experiment started at 3:00PM. We had a few false starts due to behavior cameras. Motion correction lost the lock at ~ 3:36pm but restored within ~10 seconds Andrew estimated probably 30s worth of data lost to motion artifacts in DMD2.

  Stimulus ended on time at around 4:05. Mice was removed from head fixation right away. No signs of stress. All 3 cameras flow recorded properly. We noted the camera data was stretched a bit vertically. THis could be caused by the file saving code.

Data Access

• DANDI Repository: The data from this experiment has been uploaded to the DANDI Archive and is available at: DANDI:001424/sub-796630

• Data Format: Data is stored in Neurodata Without Borders (NWB) format.

• Analysis Code: To explore the structure of the NWB file and validate its contents, you can use the validate_nwb_slap2.py script in our repository.

Disclaimer: Please note that this is an early-stage data release. The data organization, metadata structure, and analysis tools are still being refined. If you encounter any issues or have questions about the data, please open an issue in the GitHub repository.