Experiment Session Notes

• Mouse ID: 796630
• Date: 2025-09-26
• Experimenter: @amshelton @jeromelecoq
• Rig / Setup ID: SLAP2
• Stimulus version: https://github.com/AllenNeuralDynamics/openscope-community-predictive-processing/blob/d17241b1abd9c3f187c6edaac62491f724ace96e/code/stimulus-control/src/Standard_oddball_slap2_version2.bonsai
• Protocol followed: Glutamate (Green fluorophore) imaging, 65 min experiment

• Notes & Issues:

  • Same experiment as last time. We record 2 behavior videos (eye, face) with HARP timestamps aligned with the other events.
  • Behavior camera broke during set-up (the connector disconnected inside). This delayed our start.
  • SLAP2 was ran with SLAP trials to avoid analysis pipeline issues (30 seconds each, longer than before).
  • Screen is Gamma calibrated.
  • This design : https://github.com/AllenNeuralDynamics/openscope-community-predictive-processing/discussions/98
  • Orientation 1 : 60 trials
  • Oddball block 1 : Standard 0deg (1443 trials), 45 and 90 oddballs, omission (30 trials) shuffled, Oddball occurs on average every 11s
  • Orientation 2 : 33 trials
  • Oddball block 2 : Standard 0deg (1443 trials), 0 and 45 oddballs, omission (30 trials) shuffled, Oddball occurs on average every 11s
  • Orientation 3 : 33 trials
  • Nb Receptive fields : 10

  Mouse head fixed at 9:50PM

  Total Expected duration = 65 min

  Male mouse is labelled with 4E12 ExP32222-hSyn-Flex-iGluSnFR4.v9601.NGR + 2E12 pAAV.CAG.Flex.RCAMP3.WPRE.SV40 + 6E8 AAV9-CamKII0.4-Cre-SV40 injected on 4/1/2025 in V1, DOB 01/21/2025 We will image GluSNF4 with two photon.

  Andrew circled few proximal dendrites and apical dendrites + a band in soma.

  No issues throughout the experiment. Mouse is not stressed.

  Experiment started at about 10:50PM. Dense expression found at the following coordinates relative to bottom of the window:

  X: -181.12, Y: -190.5, Z: 0.0

  took larger (41 plane) ref stack to capture soma and dendrites

  Imaged at 75% power

  DMD1 depth = 140um and DMD2 depth = 160um below the pia. Exact depth of dendrites shoudl be checked in metadata.

  Motion correction had difficulty on DMD1 Motion correction on DMD1 lost completely at one point ~50min into session but recovered soon after

  Stimulus ended on time. Mice was removed from head fixation right away. No signs of stress. 2 cameras flow recorded.

Data Access

• DANDI Repository: The data from this experiment has been uploaded to the DANDI Archive and is available at: DANDI:001424/sub-796630

• Data Format: Data is stored in Neurodata Without Borders (NWB) format.

• Analysis Code: To explore the structure of the NWB file and validate its contents, you can use the validate_nwb_slap2.py script in our repository.

Disclaimer: Please note that this is an early-stage data release. The data organization, metadata structure, and analysis tools are still being refined. If you encounter any issues or have questions about the data, please open an issue in the GitHub repository.