Experiment Session Notes

• Mouse ID: 796630
• Date: 2025-09-26
• Experimenter: @amshelton @jeromelecoq
• Rig / Setup ID: SLAP2
• Stimulus version: https://github.com/AllenNeuralDynamics/openscope-community-predictive-processing/blob/d17241b1abd9c3f187c6edaac62491f724ace96e/code/stimulus-control/src/Standard_oddball_slap2_version2.bonsai
• Protocol followed: Glutamate (Green fluorophore) imaging, 65 min experiment

• Notes & Issues:

  • Same experiment as last time. We record 2 behavior videos (eye, face) with HARP timestamps aligned with the other events.
  • Behavior camera broke during set-up (the connector disconnected inside). This delayed our start.
  • SLAP2 was ran with SLAP trials to avoid analysis pipeline issues (30 seconds each, longer than before).
  • Screen is Gamma calibrated.
  • This design : https://github.com/AllenNeuralDynamics/openscope-community-predictive-processing/discussions/98
  • Orientation 1 : 60 trials
  • Oddball block 1 : Standard 0deg (1443 trials), 45 and 90 oddballs, omission (30 trials) shuffled, Oddball occurs on average every 11s
  • Orientation 2 : 33 trials
  • Oddball block 2 : Standard 0deg (1443 trials), 0 and 45 oddballs, omission (30 trials) shuffled, Oddball occurs on average every 11s
  • Orientation 3 : 33 trials
  • Nb Receptive fields : 10

  Mouse head fixed at 9:50PM

  Total Expected duration = 65 min

  Male mouse is labelled with 4E12 ExP32222-hSyn-Flex-iGluSnFR4.v9601.NGR + 2E12 pAAV.CAG.Flex.RCAMP3.WPRE.SV40 + 6E8 AAV9-CamKII0.4-Cre-SV40 injected on 4/1/2025 in V1, DOB 01/21/2025 We will image GluSNF4 with two photon.

  Andrew circled few proximal dendrites and apical dendrites + a band in soma.

  No issues throughout the experiment. Mouse is not stressed.

  Experiment started at about 10:50PM. Dense expression found at the following coordinates relative to bottom of the window:

  X: -181.12, Y: -190.5, Z: 0.0

  took larger (41 plane) ref stack to capture soma and dendrites

  Imaged at 75% power

  DMD1 depth = 140um and DMD2 depth = 160um below the pia. Exact depth of dendrites shoudl be checked in metadata.

  Motion correction had difficulty on DMD1 Motion correction on DMD1 lost completely at one point ~50min into session but recovered soon after

  Stimulus ended on time. Mice was removed from head fixation right away. No signs of stress. 2 cameras flow recorded.