Experiment Session Notes

• Mouse ID: 801381
• Date: 2025-06-05
• Experimenter: @Seyedolmohadesin @jeromelecoq
• Rig / Setup ID: SLAP2
• Stimulus version: https://github.com/AllenNeuralDynamics/openscope-community-predictive-processing/blob/2d33ef77ce2d7277fbaf42f7ee7922baa461b3ae/code/stimulus-control/src/Standard_oddball_slap2.bonsai
• • Protocol followed: Glutamate (Green fluorophore) + Calcium (Rcamp3 red fluorophore) imaging, 50 min experiment

• Notes & Issues:

  • Same experiment as last time. We record behavior videos (eye, face) with HARP timestamps aligned with the other events. Body camera was turned off as we are having file closing issues for all 3 cameras at the same time. We will fix later on.
  • SLAP2 was ran in TRIAL mode, this means we now have a continuous recording, no more SLAP trials.

  • Screen is now Gamma calibrated. It was NOT before.

  • Baseline: 2405 -> NbBaselineGrating * (1 * (0.343+0.343)) -> 27.49 min

  • Mismatch: 40 -> NbMismatchPerCondition * 4 * (0.343+0.343)) -> 1.829 min of oddballs
  • Mismatch during orientation tuning: 40 -> 14.63 min
  • Receptive field: 10 -> NbReceptiveFieldRepeats * ((0.25) * 149) -> 6.2 min of RF

  Total Expected duration = 50.16 min Oddball occurs on average every 11s

  • Head fixation started at about 2:00

Mouse is labelled with GluSNF4 and Rcamp3 both with viruses injection. Injection is rather dense. with both color present. We will image GluSNF4 and RCamp3 both with two photon. Expression is rather dense, causing a little background signal.

- Experiment started at about 2:55

Maedeh circled the soma, a few proximal dendrites and an apical dendrites. So this experiment should yield calcium (red) + synaptic inputs (green). - Proximal dendrites/soma is imaged in DMD1, Apical in DMD2, depth about 110 um for soma. - We ended on time at 3:49 - We sill need sub-optimal online motion correction on DMD1 (the plane with somas). This was better than the previous session but still not as good as the more superficial plane.