Experiment Session Notes

  • Mouse ID: 809006
  • Date: 2025-08-22
  • Experimenter: @amshelton @jeromelecoq
  • Rig / Setup ID: SLAP2
  • Stimulus version: https://raw.githubusercontent.com/AllenNeuralDynamics/openscope-community-predictive-processing/eb4f723e4dbb7725d0e0f65a45f615072f8b69d8/code/stimulus-control/src/Standard_oddball_slap2_version2.bonsai NOTE AGAIN (Forgot to commit): We fixed a bug in the oddball block to make sure standard orientation alternated. Not On github yet. The code fix bug with camera file closure and fake initial pulses sent to SlAP.
    • Protocol followed: Glutamate (Green fluorophore) imaging, 65 min experiment
  • Notes & Issues:

    • Same experiment as last time. We record behavior videos (body, eye, face) with HARP timestamps aligned with the other events.
    • Mouse has very few cells labelled. We only found 2 neurons. We are not sure why. Expression is also dimmer than previously.
    • SLAP2 was ran with SLAP trials to avoid analysis pipeline issues (30 seconds each, longer than before).
    • Screen is Gamma calibrated.
    • This design : https://github.com/AllenNeuralDynamics/openscope-community-predictive-processing/discussions/98
    • Orientation 1 : 60 trials
    • Oddball block 1 : Standard 0deg (1443 trials), 45 and 90 oddballs, omission (30 trials) shuffled, Oddball occurs on average every 11s
    • Orientation 2 : 33 trials
    • Oddball block 2 : Standard 0deg (1443 trials), 0 and 45 oddballs, omission (30 trials) shuffled, Oddball occurs on average every 11s
    • Orientation 3 : 33 trials
    • Nb Receptive fields : 10

    EXPERIMENT WAS ABORTED. EXPRESSION LEVEL IS TOO WEAK.